Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using neat serum) with Chemiluminescence technique.
The obese protein is predicted to be secretory protein with a molecular weight of ~ 16kDELISA: 1:10,000-50,000; 10-100ng of Leptin protein/well).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：Leptin is very conserved in various species. Antibodies made to whole human leptin protein crossreacts with mouse, human and rat leptin. Antibody crossreactivity in various other species is not tested
