Animal models of human aging which display the characteristic diseases associated with aging provide insight into the cause of such diseases.
Recently a transgenic mouse model, called KLOTHO, with several premature aging phenotypes has been described.
They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span.
KL protein associated with the KLOTHO mutation is a 1014 amino acid long peptide.
It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane.
kl gene expression was observed to be tissue specific.
Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway.
KL has been reported to be localized on the cell surface when expressed on CHO cells.
Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10ug/ml using ECL
Immunohistochemistry: 2-20ug/mlOptimal dilutions to be determined by the researcher.
Control Peptide:K1893-03: KLOTHO, Mouse, Control Peptide (KL)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized powder from PBS, 0.1% BSA.
Specificity：Recognizes mouse KLOTHO (KL). No significant homology with other known proteins.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 17aa near the C-terminus, cytoplasmic domain of mouse KLOTHO, conjugated to KLH. Species Sequence Homology: human; 82%, rat; 76%.
