84-1045-45　Klotho (KL) 50ul　K1893-01

特徴

• Animal models of human aging which display the characteristic diseases associated with aging provide insight into the cause of such diseases.
• Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described.
• They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span.
• KL protein associated with the Klotho mutation is a 1014 amino acid long peptide.
• It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane.
• The extracellular domain has two internal repeats, KL-1 and KL-2 which have 20-40% sequence identity to bacterial beta-glucosidase of plants and animals and mammalian lactase glycosylceramidase suggesting a role for Klotho in sphingolipid metabolism.
• kl gene expression was observed to be tissue specific.
• Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway.
• KL has been reported to be localized on the cell surface when expressed on Cho cells.
• Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not been tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:K1893-03: Klotho, Mouse, Control Peptide (KL)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.