Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot:1-10ug/ml for affinity pure or 1:500:1:000 for neat serum using ECLELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：Human sequence is found in both alternatively spliced forms of the human KCC3 (1099 aa) and KCC3a (1150 aa) (refs 1). We have raised another antibody using the N-terminal peptide sequence that is found in the short form of hKCC3 (1099aa). Has no significant sequence homology with other KCCs or CCC. Antibody crossreactivity in various species is not established. We recommend the use of control peptide
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 17-aa peptide sequence within the cytoplasmic, C-terminus of human KCC3a
