IntroductionChloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions.
Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
The K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes.
This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across the kidney epithelia.
The net direction of this transport is out of the cells.
At least four isoforms of KCC identified (KCC1, KCC2, KCC3, and KCC4) have been identified and functionally characterized.
KCC are predicted to have the same protein structure: 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N and C-termini..
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot:1-10ug/ml for affinity pure or 1:500:1:000 for neat serum using ECL.
ELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity：Human sequence is found in one of the alternatively spliced form of the human KCC3 (1099 aa; Hiki K et al (1999). The other form of the human KCC3a (1150 aa; Mount DB et al 1999) does not share this sequence. We have raised another antibody using the C-terminal peptide sequence that is found in both forms of KCC3. Has no significant sequence homology iwith other KCCs or CCC. Antibody crossreactivity in various species is not established. We recommend the use of control peptide to establish antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：An 19-aa peptide sequence within the cytoplasmic, N-terminus of human KCC3 (1099aa; Hiki et al, 1999) was used to generate anti-KCC3. The peptide was coupled with KLH (carrier protein)
