Chloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions.
Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
The K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes.
This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across the kidney epithelia.
The net direction of this transport is out of the cells.
At least four isoforms of KCC identified (KCC1, KCC2, KCC3, and KCC4) have been identified and functionally characterized.
KCC are predicted to have the same protein structure: 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N and C-termini.
KCC3 is ~77% identical to KCC1.
It is highly expressed in brain, heart, skeletal muscle, and kidney.
KCC3 is modulated by VEGF and TNF-alpha.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:250-1:500.
Band seen at 95-110kD (brush border membrane)
ELISA: 1:5000-50,000.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:K0130-02, KCC3, Human Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 3 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%.
Specificity：Recognizes human KCC3 and KCC-3b spliced form.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 17aa sequence within the cytoplasmic, N-terminal domain of the human KCC3 (KLH). Species Sequence Homology: rat-89%, mouse-78% (KCC3b).
