Chloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions.
Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
The K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes.
This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across kidney epithelia.
The net direction of this transport is out of the cells.
At least four isoforms of KCC have been identified (KCC1, KCC2, KCC3 and KCC4) and functionally characterized.
KCC are predicted to have the same protein structure: 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N- and C-termini.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:K0120: KCC2, Rat, Control Peptide
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat KCC2. No significant sequence homology is seen with other KCCs or CCC. Species Sequence Homology: Human and mouse - 94%.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：An 18aa peptide sequence within the cytoplasmic, N-terminus of rat KCC2 was used to generate anti-KCC2.
