Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting1-10ug/ml for affinity pure or 1:500:1:000 for neat serum using Chemiluminescence technique
ELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Affinity purified over the control-peptide Sepharose
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity：Sequence is 100% conserved in human, rat, mice, porcine and rabbit. No significant sequence homology is seen with other KCCs or CCC. Antibody crossreactivity in various species is not established. We recommend the use of control peptide to establish antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Po Rb Rt
Immunogen：An 21-aa peptide sequence within the cytoplasmic, N-terminus of rat KCC1 (1) was used to generate anti-KCC1. The peptide was coupled with KLH (carrier protein)
