84-1045-31　［取扱停止］Iron Regulatory Protein (IRP-2) 100ul　I8615-03

特徴

• Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development.
• However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species.
• Humans also have very limited capacity to excrete iron.
• Therefore, cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels at the point of absorption in the intestine and other tissue.
• Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs), iron transporter (NRMAP2/DMT1/DCT1) etc play a key role in iron metabolism.
• Some genes involved in iron-metabolism are associated with genetic disorders such as Friedreich's Ataxia (Frataxin), genetic hemochromatosis (HFE), and Sex-linked anemia (Hephaestin).
• Iron regulatory proteins (IRP-1 and IRP-2) are cytoplasmic mRNA-binding proteins that control intracellular iron levels by regulating the translation of ferritin, Tfrs, and other proteins congaing iron-responsive element (IRE) located at the 5'-UTR.
• IRP binds to IREs with high affinity in situation of iron starvation.
• Binding of IRP to IRE of ferritin represses its transcription.
• However, when cells are iron replete, IRPs lose their capacity to bind IREs, allowing efficient translation of ferritin and reducing Tfr mRNA half-life.
• Although IRP1 and IRP-2 share significant protein sequence homology, they differ in tissue distribution and mode of regulation.
• IRP-1, also known as IRE-BP-1/aconitase/citrate hydrolyase/ferritin repressor protein, (mouse/rat/human 889aa, chromosome 9, ~98kD) also posses aconitase activity and highly homologous with mitochondrial aconitase.
• IRP-1 is more abundant than IRP-2 and it is widely expressed.
• IRP-2/IRE-BP2 (rat/human 963aa) is relatively less abundant than IRP-1 andl acks aconitase activity.
• It has a unique 73aa insertion in the N-terminus.
• In the presence of high iron levels IRP-2 is rapidly targeted to proteasome-mediated degradation.
• Although, both IRPs bind the consensus IREs, it is also shown that IRP-2 can recognize an exclusive IRE subset.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: ~1:100,000 using 50-100ng control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control peptide:I8670-21: Iron Regulatory Protein, Rat (IRP-2)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.