Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development.
However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species.
Humans also have very limited capacity to excrete iron.
Therefore, cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels at the point of absorption in the intestine and other tissue.
Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs), iron transporter (NRMAP2/DMT1/DCT1) etc play a key role in iron metabolism.
Some genes involved in iron-metabolism are associated with genetic disorders such as Friedreich's Ataxia (Frataxin), genetic hemochromatosis (HFE), and Sex-linked anemia (Hephaestin).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:I8670-20: Iron Regulatory Protein, Rat, Control Peptide (IRP-1
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide
Specificity：Recognized rat IRP-1. No significant sequence homology exist with IRP-2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 20aa within the N-Terminal of rat IRP-1, conjugated to KLH. Species Sequence Homology: mouse; 94%, human and rabbit; 89%
