84-1045-28　［取扱停止］Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165) 100ul　I8601

特徴

• IRAP (also known as placental leucine aminopeptidase, PLAP; leucyl cystinyl aminopeptidase, LNPEP; oxytocinase, Otase; vesicle protein of 165kD or vp165) belongs to the peptidase M1 family.
• IRAP (mouse 966aa, rat 1025 aa; human 1025 aa, chromosome 5q14.2-q15), a zinc-containing metalloprotein, degrades peptide hormones such as oxytocin, vasopressin, and angiotensin III, and inactivates Met-enkephalin and dynorphin.
• It is a type 2 membrane protein and is secreted due to proteolytic processing.
• IRAP is glycosylated and alternatively spliced to at least 3 isoform (1-15 aa missing in isoform 2 and 1-20 missing in isoform 3).
• The N-terminus (1-110 aa) is located in the cytoplasm, followed by TM domain (111-131 aa); the C-terminus (132-1025 aa) is extracellular.
• The secreted form is cleaved at 154-155 aa (processed secreted form 155-1025 aa).
• IRAP is highly expressed in placenta, heart, kidney, and small intestine.
• In brain, only the membrane form is found.
• The brain form is found to be 140kD due to differential glycosylation.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 ECL
• ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Control Peptide: I8601-01A.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.