84-1045-25　Iceberg 100ul　I0620

特徴

• Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc.
• Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine Aspartase).
• The initiator caspases can be activated by self-cleavage.
• The effector caspases are then activated in an amplification cascade.
• Caspase-1 (interleukin-1 converting enzyme (ICE)) is a cytoplasmic protease that converts 34kD inactive precursor IL-1 to the mature 17kD proinflammatory cytokine.
• Caspase-1 is synthesized as a single-chain polypeptide zymogen consisting of an N-terminal prodomain, and a large (p20) and a small (p10) catalytic domains.
• A serine/threonine kinase RIP2/CARDIAK/RICK binds caspase-1 and promotes its processing.
• RIP2 engages caspase-1 through a direct protein-protein interaction involving corresponding caspase recruitment domains (CARDs) present at the C terminus of RIP2 and within the prodomain of caspase-1.
• It is now shown that activation of caspase-1 is regulated by a small CARD-containing decoy molecule termed ICEBERG.
• This decoy protein binds the corresponding CARD motif of caspase-1, inhibiting and/or displacing the upstream activator RIP2.
• Structurally, ICEBERG has resemblance to the death-domain-fold superfamily.
• Human Iceberg is a 90-aa protein (11q21-q22).
• It is 52% identical to the caspase-1 CARD.
• It is primarily expressed in the heart and placenta.
• The related CARD-containing molecules caspase-1 and RIP2 are also expressed in the heart and placenta as well as in numerous other tissues.
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
• The predicted size of iceberg is ~ 10kD
• ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50,000 for neat serum and 0.5-1ug/ml for affinity pure)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.