Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitters.
It plays an important role in a multitude of physiological processes in central and peripheral tissues.
Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g.
In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells.
Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system.
Histamine effects are mediated by four pharmacologically distinct receptors, the H1R, H2R , H3R and H4R receptors, which are all members of the G-protein coupled receptor (GPCR) family.
Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length.
Most recently, a novel orphan G-protein coupled receptor, named H4R (GPRv53, human 390 aa) has been cloned and characterized.
It is most closely related to H3R (~37% homology).
Unlike H3R, H4R has a distinct tissue distribution and it is localized in the peripheral blood leukocytes, spleen, thymus and colon.
Mammalian cells expressing H4R were demonstrated to bind and respond to histamine in a concentration-dependent manner.
In functional assays, an H3 receptor agonist, R-(a)-methylhistamine, but also a H3 receptor antagonist, clobenpropit, and a neuroleptic, clozapine, activated H4R-expressing cells.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity：The human peptide sequence is unique to human H4R. No significant homology exists with other histamine receptors. Antibody crossreactivity in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 17 aa peptide within the cytoplasmic, C-terminus of human H4R (1) conjugated to KLH
