84-1045-09　［取扱停止］Histamine Receptor 3 (H3R) 100ul　H5090-20B

特徴

• Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitter.
• It plays an important role in a multitude of physiological processes in central and peripheral tissues.
• Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g.
• sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation).
• In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells.
• Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system.
• Histamine effects are mediated by four pharmacologically distinct receptors: the H1R, H2R , H3R and H4R receptors.
• These receptors are all members of the G-protein coupled receptor (GPCR) family.
• Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length.
• Human Histamine 3 receptor, H3R (GPCR97), has recently been identified as a presynaptic autoreceptor on histamine neurons in the brain controlling the stimulated release of histamine.
• The H3 receptor has been shown to be a presynaptic heteroreceptor in nonhistamine- containing neurons in both the central and peripheral nervous systems.
• H3R (rat/human 445aa, ~93% homology) is primarily expressed in the brain.
• Several studies using H3 selective agonists revealed that H3R couples to pertussis toxin-sensitive Gi/o protein.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL.
• ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.
• Optimal dilutions to be determined by the researcher.
• Control Peptide: H5090-20A
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.