Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitters.
It plays an important role in a multitude of physiological processes in central and peripheral tissues.
Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g.
In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells.
Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system.
Histamine effects are mediated by four pharmacologically distinct receptors, the H1R, H2R , H3R and H4R receptors, which are all members of the G-protein coupled receptor (GPCR) family.
Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length.
The H2R (mouse/rat 358 aa; human 359 aa chromosome 5) causes cAMP accumulation in the gastric cells, cardiac tissues, smooth muscle cells and immune cells.
H2R agonists have been proven to be effective for acid peptic disorders of the GI tract.
H2R is moderately expressed in the brain.
H2 receptor agonist, dimaprit, is a relatively selective H2 receptor agonist with minimal H1R receptor agonism.
Recently, amthamine (2-amino-5-(2-aminoethyl)-4-methylthiazole), a rigid dimaprit analog, combines a high H2R receptor selectivity.
Interestingly, mutations in H2R has been linked to schizophrenia.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity：The peptide sequence is 100% conserved in mouse H2R. No significant homology exists with human H2R or other histamine receptors. For human, We recommend antibody feline # H2R22 made against the human H2R sequence. Antibody crossreactivity in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：A 14 aa peptide within the cytoplasmic C-terminus of Rat H2R (1) conjugated to KLH
