Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitter.
It plays an important role in a multitude of physiological processes in central and peripheral tissues.
Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions.
In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells.
Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system.
Histamine effects are mediated by four pharmacologically distinct receptors, the H1R, H2R , H3R and H4R receptors, which are all members of the G-protein coupled receptor (GPCR) family.
Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length.
H1R is distributed in the brain, most smooth muscle cells, endothelial cells, adrenal medulla, and heart.
H1R plays roles in smooth muscle contraction, stimulation of nitric oxide formation, endothelial cell contraction, and increasing vascular permeability, all of which have close relationships with allergic conditions.
H1R preferentially couples to the Gq/11 family of G-proteins and causes mobilization of intracellular Ca 2+ in a pertussis toxin-insensitive fashion.
Compounds such as mepyramine (pyrilamine) and triprolidine are highly potent H1 antagonists, and easily penetrate the brain causing sedation.
Many new non-sedating H1 antagonists.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-1:100,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes rat H1R. No significant sequence homology exists with other histamine receptors. Species Crossreactivity: mouse and bovine.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Bo Mo Rt
Immunogen：A 17 aa peptide near the cytoplasmic, C-terminus of rat H1R. Conjugated to KLH. Species sequence homology: 94% in human, fish, monkey and 80% in guinea pig.
