Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitters.
It plays an important role in a multitude of physiological processes in central and peripheral tissues.
Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g.
In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells.
Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL
ELISA: 1:10,000-1:100,000 (Coat with 50-100ng of control peptide (H5090-01B)/well)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes rat histamine 1 receptor 1. No significant sequence homology exists with other histamine receptors. Species sequence homology: mouse and bovine; 100%, 94%; human, fish and monkey and 80%; guinea pig
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 17 aa peptide near the cytoplasmic, C-terminus of rat H1R (KLH).
