Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins.
Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver.
Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide.
The signal peptide is cleaved to produce pro-HEPC, which is further processed to generate the mature secreted form of hepcidin (C-terminal 20, 22 or 25-aa HEPC).
In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities.
The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus.
The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice.
The human gene is located at chromosome 19, in close proximity with Usf2 gene.
Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice.
Hepcidins are devoid of IRE.
Like other antimicrobial peptides, hepcidin is up-regulated by lipopolysaccharides (LPS).
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff.
pure IgG using ECL
ELISA: ~1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control peptide, because of the small size (2-3kD), is not recommended for Western.
It should be used in ELISA or antibody blocking (use 5-10ug control peptide per 1ug of IgG or 1ul of antiserum) experiments to demonstrate antibody specificity.
Full length, human 25-aa, 20-aa HEPC and mouse 25-aa HEPC are also available for various studies.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The human sequences is 63% conserved in mouse/rat pro-HEPC. This sequence is removed from the mature 20-25 secreted forms of HEPC. So antibodies will not react with the secreted forms of HEPC.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：An 11-aa peptide sequence from the N-terminus (after the signal peptide) of human Pro-HEPC was synthesized, conjugated to KLH.
