Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins.
Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver.
Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide.
The signal peptide is cleaved to produce pro-HEPC, which is further processed to generate the mature secreted form of hepcidin (C-terminal 20, 22 or 25-aa HEPC).
In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities.
The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus
The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice.
The human gene is located at chromosome 19, in close proximity with Usf2 gene.
Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice.
Hepcidins are devoid of IRE.
Like other antimicrobial peptides, hepcidin is up-regulated by lipopolysaccharides (LPS)
An 11-aa peptide sequence from the N-terminus (after the signal peptide) of mouse Pro-HEPC was synthesized, conjugated to KLH and antibodies generated in Rabbits.
Control peptide was also used to affinity purify antibodies.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml ELISA: ~1:100K using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:H2009-01A Hepcidin, pro, Human, Control Peptide (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes mouse Hepcidin, pro. Species sequence homology: rat 91% and in human 63% pro-HEPC. This sequence is removed from the mature 20-25 secreted forms of HEPC.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：An 11-aa peptide sequence from the N-terminus (after the signal peptide) of mouse Pro-HEPC was synthesized, conjugated to KLH.
