84-1044-85　Hepcidin, CT, 13aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) 100ul　H2008-53

特徴

• Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins.
• Hepcidin (unprocessed, pro-protein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver.
• Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide.
• The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues.
• The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice.
• The human gene is located at chromosome 19, in close proximity with Usf2 gene.
• Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice.
• Hepcidins are devoid of IRE.
• Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilutions:ELISA: ~1:100,000 using 50-100ng control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:H2008-55: Hepcidin, Mouse, 13aa, CT, Control Peptide
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.