Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway.
Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter and iron.
Biliverdin is subsequently converted to bilirubin by biliverdin reductase.
These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs.
To date, 3 forms of heme oxygenases (HO1-3) have been identified.
A third enzyme, HO-3 (rat 290aa ~ 33kD) has been cloned and characterized from rat brain.
The HO-3 mRNA is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis.
The predicted amino acid structure of HO-3 differs from both HO-1 and HO-2 but is closely related to HO-2 (~ 90% similarity).
HO-3 sequence has two HRM known to be involved in heme binding.
It is a poor heme catalyst.
Therefore, HO-3 has a potential regulatory role in heme-dependent cellular processes.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:H1847-97: Heme Oxygenase 3, Rat (HO-3) Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes rat Heme Oxygenase 3 (HO-3). The rat sequence is not well conserved in HO-1 or HO-2.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 11aa from the N-terminus of rat Heme Oxygenase 3 (HO-3).
