Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway.
Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter, and iron.
Biliverdin is subsequently converted to bilirubin by biliverdin reductase.
These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs.
To date, 3 forms of heme oxygenases (HO1-3) have been identified.
A third enzyme, HO-3 (rat 290 aa ~ 33kD) has been cloned and characterized from rat brain.
The HO-3 mRNA is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis.
The predicted amino acid structure of HO-3 differs from both HO-1 and HO-2 but is closely related to HO-2 (~ 90% similarity).
HO-3 sequence has two HRM known to be involved in heme binding.
It is a poor heme catalyst.
Therefore, HO-3 has a potential regulatory role in heme-dependent cellular processes.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff.
pure IgG using ECL.
HO-2 ~32-34kD.
ELISA: ~1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The rat sequence is not well conserved in HO-1 or HO-2. HO-3 sequences form other species are not yet available. It is not clear if HO-3 is expressed in various tissues and species. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISAor antibody blocking (use 5-10 ug control peptide per 1 ug of IgG or 1 ul of antiserum) experiments to demonstrate antibody specificity.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：An 11-aa peptide sequence within the N-terminus of rat HO-3 (1) was synthesized, coupled to KLH
