Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway.
Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter, and iron.
Biliverdin is subsequently converted to bilirubin by biliverdin reductase.
These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs.
To date, 3 forms of heme oxygenases (HO1-3) have been identified.
HO-2 (mouse/rat 315 aa; human 316 aa; chromosome 16) is non-inducible form.
Except for corticosterone, no other inducer of HO-2 has been identified to date.
HO-2 has been localized to endothelial cells and adventitial nerves of blood vessels.
It is also localized to neurons in autonomic ganglia, including the petrosal, superior cervical, and nodose ganglia, as well as ganglia in the myenteric plexus of the intestine.
Low levels of Ho-2 are found in most tissues except spleen.
At the amino acid level,HO-1 and HO-2 are 42% homologous.
However, each isozyme is evolutionary highly conserved.
The molecular weight of mammalian HO-1 is ~31-33kDa and HO-2 is ~36kDa.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff.
pure IgG using ECL.
HO-2 ~32-34kD
ELISA: ~1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control peptides, because of the small size (2-3kD), is not recommended for Western.
It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1 ug of IgG or 1 ul of antiserum) experiments to demonstrate antibody specificity.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：The human sequence is 100% conserved in mouse, rat, human HO-2. Greatly conserved in HO-1. High sequence sonservation is observed in Euryoxic fish, puffer fish. Cyanobacteria and plant read Alga Heme oxygenases. So this antibody is likely to crossreact with HO-2 and HO-1 from many species. This antibody should be use useful in detecting the HO when no specificity is required.
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 26-aa peptide sequence near the middle of the Human HO-2 (1) was synthesized, coupled to KLH
