HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25.
The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels.
In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore.
By affecting the spatial coordination of the GYG sequence in the tetrameric channel complex, other channel domains could control the structural rigidity of the pore and thereby determine whether only K+ or also other monovalent are allowed to permeate.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:H1827-31F: HCN1 pore, Human (Hyperpolarization-activated Cyclic Nucleotide-gated channel Pore)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes human HCN1 pore. The pore region of HCN1 is very conserved among various HCNs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues aa225-247 near the pore region of human HCN1. Species Sequence Homology: HCN1: mouse, rat and rabbit; 100%; HCN2: mouse, rat and human; 86%; HCN3: mouse; 95%, rat; 90%; HCN4: rat and human; 95%.
