84-1044-70　HCN1 pore (Hyperpolarization-activated Cyclic Nucleotide-gated channel Pore) 100ul　H1827-31

特徴

• Biological rhythms (beating of the heart, circadian sleep cycles, respiration, and the release of hormones) are necessary to sustain life.
• Heart beating is the most reliable and rhythmic biological phenomenon.
• Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential.
• The hyperpolarization-activated cation current (termed If, Ih, or Iq) plays a key role in the in the initiation and modulation of cardiac and neuronal pacemaker depolarization.
• The generation of cardiac pacemaker potentials relies on a complex interplay between at least four different types of cation channels: T- and L-type Ca2+ channels, K+ channels, and a cation channel termed If (synonymous names are Ih and Iq).
• The If channel has been designated as "pacemaker" channel because it reveals unique features that are believed to be a prerequisite for pacemaker activity.
• Recently, the hyperpolarization-activated cyclic nucleotide-gated families of ion channel proteins (HCN1-4) have been identified as the "pacemaker" channel.
• The amino acid sequences of HCNs predict a structure similar to that of voltage-gated (Kv) channels and cyclic nucleotide-gated (CNG) channels.
• HCNs proteins (HCN1-4) are characterized by six transmembrane domains (S1-S6), including a including a positively charged voltage-sensing S4 segment and an ion-conducting pore between S5 and S6.
• In the C terminus the HCNs carry a cyclic nucleotide-binding domain (CNBD), a motif found in several cyclic nucleotide-binding proteins.
• The core region of HCNs channels (S1 to the C terminus of the CNBD) is highly conserved, whereas the cytoplasmic N and C-termini, vary considerably in their length and share only weak sequence homology.
• The functional HCN, like other members of the superfamily of voltage-gated cation channels, channels probably assemble into tetrameric complexes.
• HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25.
• The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels.
• In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore.
• By affecting the spatial coordination of the GYG sequence in the tetrameric channel complex, other channel domains could control the structural rigidity of the pore and thereby determine whether only K+ or also other monovalent are allowed to permeate.
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
• ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.