84-1044-63　HCN1 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 1) 100ug　H1827-25F

特徴

• Biological rhythms (beating of the heart, circadian sleep cycles, respiration, and the release of hormones) are necessary to sustain life.
• Heart beating is the most reliable and rhythmic biological phenomenon.
• Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential.
• The hyperpolarization- activated cation current (termed If, Ih, or Iq) plays a key role in the in the initiation and modulation of cardiac and neuronal pacemaker depolarization.
• The generation of cardiac pacemaker potentials relies on a complex interplay between at least four different types of cation channels: T- and L-type Ca2+ channels, K+ channels, and a cation channel termed If (synonymous names are Ih and Iq).
• The If channel has been designated as "pacemaker" channel because it reveals unique features that are believed to be a prerequisite for pacemaker activity.
• Recently, the hyperpolarization-activated cyclic nucleotide-gated families of ion channel proteins (HCN1-4) have been identified as the "pacemaker" channel.
• The amino acid sequences of HCNs predict a structure similar to that of voltage-gated (Kv) channels and cyclic nucleotide-gated (CNG) channels.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using ECL
• ELISA: 1:10,000-1:100,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.