The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains.
Glycine receptor exists in 2 forms-GLYT1 and GLYT2.
Form 1 has three other known isoforms (GLYT1a, GLYT1b, and GLYT1c) which may be produced by alternative splicing or promoter usage.
Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and are expressed from the same gene.
Although the N-terminal part of mouse and rat GLYT1a is identical in mouse and rat, the N-terminus of GLYT1b is significantly different in these two species.
The GLYT1c subtype has only been reported in humans.
GLYT1a is expressed in CNS and peripheral organs.
GLYT1b is localized in the CNS.
Both neuron and Glial cells have GLYT1.
More recent studies indicate that GLYt1b may not be brain specific.
Immunolocalization studies on GLYT2 suggest that GLYT2 is responsible for terminating the neurotransmission at the strychnine synapses.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-1:50,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: G8168-25
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide. Glycerol free.
Specificity：Recognizes rat GLYT2. No homology is seen with either GLYT1 or other transporters.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide consisting of 20aa sequence within the cytoplasmic, C-terminal domain of rat GLYT2. Species Sequence Homology: human and mouse.
