84-1044-56　Glycine Transporter-2 (GLYT2) 100ul　G8168-21

特徴

• Glycine is one of the major inhibitory neurotransmitters in the spinal cord and brain stem.
• Glycine acts on the strychnine-sensitive glycine receptors, a pentameric Cl-channel, to generate inhibitory postsynaptic potentials.
• Glycine can modulate excitatory neurotransmission as an obligatory co-agonist with glutamate at NMDA-activated glutamate receptors via binding site on the NMDA receptor, which is distinct from the strychnine-sensitive glycine receptor-binding site.
• Glycinergic synapses are primarily present throughout the spinal cord, brain stem and cerebellum, as well as in the retina and a few other brain regions.
• Neurotransmitter action is terminated by re-uptake of glycine via specific high affinity transporter protein located at the plasma membrane of neuron and glial cells.
• The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains.
• Glycine receptor exists in 2 forms (GLYT1 and GLYT2).
• Form 1 has three other known isoform (GLYT1a, GLYT1b and GLYT1c) which may be produced by alternative spicing or promoter usage.
• Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and expressed from the same gene.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL)
• ELISA: 1:10,000-1:50,000.
• Coat with 50-100ng control peptide/well
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:G8168-25: Glycine Transporter-2, Rat, Control Peptide (GLYT2)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.