84-1044-55　Glycine Transporter-1 (GLYT1) 100ul　G8168-04

特徴

• The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains.
• Glycine receptor exists in 2 forms-GLYT1 and GLYT2.
• Form 1 has three other known isoforms (GLYT1a, GLYT1b, and GLYT1c) which may be produced by alternative spicing or promoter usage.
• Rodent Glyt1a and Glyt1b differ only by 10 amino acid at the N-terminus and expressed from the same gene.
• Although, the N-terminal part of mouse and rat GLYT1a is identical in mouse and rat, the N-terminus of GLYT1b are significantly different in these two species.
• The GLYT1c subtype has only been reported in humans.
• GLYT1a is expressed in CNS and peripheral organs.
• GLYT1b is localized in the CNS.
• Both neuron and Glial cells have GLYT1.
• More recent studies indicate that GLYt1b may not be brain specific.
• Immunolocalization studies on GLYT2 suggest that GLYT2 is responsible for terminating the neurotransmission at the strychnine synapses.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
• ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.
• Immunohistochemistry & Immunofluorescence: We recommend the use of affinity purified antibody at 10-50ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.