84-1044-52　Glutathione S-Transferase pi (GSTpi) 100ul　G8135-26H

特徴

• GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens.
• The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation.
• GSTs play an important role defense against electrophilic chemicals generated by cellular oxidative reactions catalyzed by cytochrome P450 and other oxidases.
• GSTs are believed to defend against the highly reactive electrophiles that are formed as a result of cellular oxidative processes.
• Based on amino acid sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma.
• GSTs exists as homodimers of ~25kD.
• GST A1-1 is mainly found in kidney, intestine, lung, and liver.
• GST M1-1 is restricted to liver and few other tissues.
• GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:100,000.
• Coat ELISA plates with 50-100ng/well GST pi.
• Western Blot: 1:500-1:3000 (ECL) Note: Actual dilution of antibody will vary depending upon the type of technique used to detect primary antibody such colorimetric or chemiluminescence substrates.
• The nature and concentration of GST in may also affect antibody concentration
• Optimal dilutions to be determined by the researcher.
• Western Blot Positive Control:G8135-26D
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.