84-1044-46　［取扱停止］Glutamate Transporter EAAT2 (GLT1) 100ul　G4005-91B

特徴

• Glutamate is the main excitatory neurotransmitter in the brain.
• To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4 and EAAT5.
• These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells.
• Glutamate transporters (525-573aa) display about 55% homology and are predicted to contain up to 6-10 transmembrane domains.
• Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord.
• EAAC1 is specific for certain neurons and Purkinje cells.
• It is specifically enriched in cortex, hippocampus and caudate-putamen.
• it is confined to presynaptic and postsynaptic elements.
• GLAST has been observed in both neuron and astroglia.
• It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum.
• EAAT4 has properties of ligand gated Cl-channel.
• It is localized mainly in cerebellar Purkinje cells in rat and human CNS.
• EAAT5 has only been cloned from human.
• It is primarily expressed in retina.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:10,000-1:50,000.
• Coat plates with 1ug/ml Control Peptide per well.
• Optimal dilutions to be determined by the researcher.
• Control Peptide: G4005-91A
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.