Glutamate is the main excitatory neurotransmitter in the brain.
To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5.
These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells.
Most recently, vesicular type transporters for glutamate, termed VGLUTs (VGLUT1/BNPI, VGLUT2/DNPI, and EAT-4), have been cloned and characterized that are related to phosphate transporters.
Although neurons exhibit Na-dependent Pi transport, the biological role of Pi uptake is not clear.
Proximal tubules in the kidney reabsorb Pi in the glomeruli by the action of a group of phosphate transporters (Type 1-NaPi related, type 2-NaPi-2 related, and type 3-viral receptor-related.
These receptors show weak (~20% identity) between various subtypes.
A distinct type of brain specific Na+-dependent phosphate (Pi) transporter (BNPI), originally characterized as a plasma membrane transporter has been localized in a subset of glutamatergic neurons (amygdla,cereberal cortex, and hippocmaplus) and identified as VGLUT1 (rat and human 560 aa; ~60kD, ~30% homology with type-1 Pi-transporters).
A novel transporter VGLUT2/DNPI (Differentiation-assocaited Na-Pi cotransporter, ~75% homology with VGLUT1; human/rat/mouse VGLUT2 582 aa) has also been implicated in vesicular glutamate transport.
It is expressed in only a subset of neurons.
It is also localized to synaptic vesicles, at synapses exhibiting classical excitatory features.
VGLUT2 mRNA is found in brain regions that lack VGLUT1.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique.
VGLUT2 is ~65kD protein (1).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：The rat peptide is 90% conserved in mouse and 70% in human VGLUT2. No significant sequence homology is observed with VGLUT1, EAT-4, or other NaPi-related (Type-1 to Type-III) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 20 AA synthetic peptide witin the C-terminus of rat VGLUT2 was synthesized, conjugated to KLH
