Glutamate is the main excitatory neurotransmitter in the brain.
To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5.
These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells.
Glutamate transporters (525-573aa) display about 55% homology and are predicted to contain up to 6-10 transmembrane domains.
Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord.
EAAC1 is specific for certain neurons and purkinje cells, and specifically enriched in cortex, hippocampus, and caudate-putamen and confined to presynaptic and postsynaptic elements.
GLAST has been observed in both neuron and astroglia.
It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum.
EAAT4 has properties of ligand gated Cl-channel.
It is localized mainly in cerebellar Purkinje cells in rat and human CNS.
EAAT5 has only been cloned from human.
It is primarily expressed in retina.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Western Blot: 1-10ug/ml using ECL
Immunohistochemistry: 2-10ug/ml for paraformaldehyde fixed sections of tissue
Optimal dilutions to be determined by the researcher.
Control Peptide:G4003-94A: Glutamate Transporter EAAT5, Human
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human Glutamate Transporter EAAT5. No significant sequence homology to other glutamate transporters.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 21aa in the cytoplasm of the C-terminus of human Glutamate Transporter EAAT5 (KLH). Species Sequence Homology: mouse; 88%, salamander; 71%
