Inhibitory protein factor (IPF) refers to a three distinct proteins with ~mol wt of 138kDa (IPF-alpha), 135kD (IPF-beta), and 132kD (IPF-gamma).
IPF-a is derived from a ubiquitous, non-erythroid brain spectrin called alpha-Fodrin, a well-characterized protein previously implicated in such diverse activities as exocytosis/endocytosis, apoptosis, and NMDA-receptor activation.
However, a-Fodrin itself has no effect on glutamate uptake.
The N-terminal 1-20 aa of IPF-a, IPF-b, and IPF-g are identical with 26-45 aa of a-Fodrin (mol wt ~240kD).
Therefore, it appears that some identified protease(s) may generate IPF-a from a-Fodrin.
IPF does not inhibit ATP-independent uptake, norepinephrine uptake into chromaffin vesicles, and Na-dependent glutamate uptake into synaptosomes.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL technique.
ELISA: 0.5-1ug/ml.
Coat plates with 1ug/ml control peptide.
Optimal dilutions to be determined by the researcher.
Control Peptide: G4000-72
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, before the addition of glycerol to 40%.
Specificity：Recognizes human IPF. Species Sequence Homology: Rat:100%; Chicken spectrin alpha chain: 90%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic 10 amino acid peptide near the N-terminus of human IPF (KLH coupled).
