Antibodies made to this epitope have been shown to recognize the 67kD MW.
Band in rat brain (2)
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: G4000-12F
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity:The 18 AA rat immunogenic peptide sequences is 100% homologous with mouse, 88% in human and 61% with monkey GAD67. Antibody crossreactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Mo Rt
Immunogen:An 18-aa peptide sequence near the amino terminus of mouse/rat GAD67 (KLH coupled).