Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum, ECL.
Antibodies made to this epitope have been shown to recognize the 67kD MW.
Band in rat brain (2)
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: G4000-12F
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Purified over control peptide-Sepharose
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 18 AA rat immunogenic peptide sequences is 100% homologous with mouse, 88% in human and 61% with monkey GAD67. Antibody crossreactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：An 18-aa peptide sequence near the amino terminus of mouse/rat GAD67 (1) was synthesized and coupled to KLH
