Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT-1 to GLUT-7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1: 662aa, ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N- and C-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
GLUT isoform differ in their tissue expression, substrate specificity and kinetic characteristics.
GLUT-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
GLUT-2 provides glucose to the liver and pancreatic cells.
GLUT-3 is the main transporter in neurons.
GLUT-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
GLUT-5 transports fructose in intestine and testis.
GLUT-6/SLC2A6, originally described as gLUT-9 has been reassigned as GLUT-6.
GlLUT-6 (human: 507aa, ~ 48kD, chromosome 9q34) is most closely related to GLUT-8 (~45% homology).
It is highly expressed in brain, spleen, and peripheral leukocytes.
GLUT-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane.
GlLUT-8 is found in adult testis and placenta.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Control peptide (Cat #G3900-97) can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity：Recognizes human GLUT 12. No significant sequence homology with other GLUTs. Species Sequence Homology: monkey: 100%; mouse: 71%; rat: 69%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide consisting of 15aa sequence near the cytoplasmic, C-terminus of Human Glut-12 (KLH coupled).
