Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW.
40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and c-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility.
It is widely expressed with highest levels in liver and pancreas.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL
ELISA: 1:10,000-50,000; Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide: G3900-95 GLUT 10, Control Peptide, Human (Glucose Transporter)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human GLUT10. Human peptide sequence has no significant sequence homology with other gluts.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 16-AA peptide sequence near the cytoplasmic, C-terminus of Human Glut-11 (1) was coupled to KLH
