Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW.
40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and c-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
Glut-2 provides glucose to the liver and pancreatic cells.
Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
Glut-5 transports fructose in intestine and testis.
Glut-6 is a pseudoegene and unlikely to be expressed at the protein level.
Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane.
Glut-8 is found in adult testis and placenta.
Human Glut-9 is expressed in spleen, peripheral leukocytes and brain.
Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility.
It is widely expressed with highest levels in liver and pancreas.
Glut-11 (496 aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure IgG using ECL.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：Mouse peptide sequence is 90% conserved in rat and human Glut-8. No significant sequence homology is seen with other transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used in ELISA or antibody blocking experiments (use 5-10 ug of control peptide per 1 ug IgG or 1 ul of antiserum) to confirm antibody specificity.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：An 11-AA peptide sequence near the C-terminus of mouse Glut-8 (1). A cysteine has been added at the NH2 terminus for coupling to KLH.
