Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family).
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, Mol.
Wt.
40-60 kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and C-termini are predicted to be cytoplasmic.
There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot:1-10ug/ml using ECL.
Detects a major band at about 52kD in various tissues (2)
ELISA: 0.5-1ug/ml, Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:G3900-80C GLUT 7, Rat Control Peptide.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in 100mM Tris, pH 7.4 and 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat GLUT 7. Species sequence homology: human. Glut-7 64%: Glut-2 68% and show similar secondary and tertiary structures. However, Glut-7 contains an extra 6 amino acid (KKMKND) at the C-terminal end. This sequence is very similar to the last 6 amino acids of some of the mircosomal UDP-glucoronosyltransferase isoenzymes (see refs. 1). The KK-K-- has been considered motif for the retention of transmembrane proteins (3) and is not found in Gluts.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 14 AA peptide sequence near the C-terminus of rat Glucose Transporter 7 (1). A cysteine has been added at the NH2 terminus for coupling to KLH.
