Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 7, MW.
40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N- and C-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting
GLUT isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
GLUT 6/SLC2A6, originally described as GLUT 9 has been reassigned as GLUT 6.
GLUT 6 (human 507aa; ~48kD; chromosome 9q34) is most closely related to GLUT 8 (~45% homology).
It is highly expressed in brain, spleen and peripheral leukocytes.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml, Control peptide (Cat# G3900-75) can be used to coat ELISA plates at 1ug/ml
Western Blot:1-10ug/ml using ECL
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human GLUT 6.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 16aa synthetic peptide sequence near the cytoplasmic, C-terminus of human GLUT 6, coupled to KLH
