84-1044-13　［取扱停止］GLUT 5 (Glucose Transporter) 100ug　G3900-65L

特徴

• Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
• Molecular cloning of these glucose transporters has identified a family ofclosely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol.
• Wt.
• 40-80 kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662 amino acids; ~75 kD).
• Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
• Both N and C-termini are predicted to be cytoplasmic.
• Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue specific targeting.
• Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
• Applications:Suitable for use in ELISA, Western Blot ,Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (ECL).
• Rat glut-5 is ~60kD in rat jejunum membranes that is slightly larger that that reported for human glut-5 (50-55kD).
• As opposed to human tissues, rat glut-5 mRNA has not been detected in rat testis, adipose tissues and skeletal muscle.
• In rat kidney, a protein of ~42kD was detected
• ELISA: 0.5-1ug/ml.
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Control peptide: G3900-65M
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.