Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
The antibody has been shown to react with a protein band of about 60kD in the rat jejunum membranes that can be competed with the control peptide (2).
The MW.
Of Glut 5 is larger than that reported for human Glut 5 (50-55kD) (3).
As opposed to human tissues, rat Glut-5 mRNA has not been detected in rat testis, adipose tissues, and skeletal muscle (2, 4).
In rat kidney, a protein of about 42kD was detected (5).
Differences in MW.
Of Glut-3 in various tissues has also been noted.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：Rat peptide sequence shows ~ 61% homology with human Glut-5 (8/13 AA). However, antibody raised to the C-terminal sequences from human Glut-5 does not crossreact with rat Glut-5 (5). For human Glut-5, we suggest the use antibodies that are made to a peptide from human Glut5. Antibody crossreactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A cytoplasmic 13 AA peptide sequence near the C-terminus of rat liver glucose/fructose transporter (Glut-5) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH
