84-1044-07　GLUT 4 (Glucose Transporter) 100ul　G3900-60A

特徴

• Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
• Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, MW:40-80 kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75 kD).
• Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
• Both N and C-termini are predicted to be cytoplasmic.
• Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
• Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
• Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
• Glut-2 provides glucose to the liver and pancreatic cells.
• Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 ELISA: 1:1000-10,000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.