Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family).
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, MW 40-60 kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and C-termini are predicted to be cytoplasmic.
There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
Major band at ~45kD
ELISA: 0.5-1ug/ml.
Coat ELISA plates at 1ug/ml (antigen)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Store long-term at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes Glut-3. The mouse Glut-3 peptide sequence used for antibody production is 100% identical in rat. This antibody is specific for mouse and rat. Does not recognize human Glut-3 because of significant interspecies differences in C-terminal Glut-3.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：A 12 aa C-terminal peptide sequence near the C-terminus of mouse glucose transporter-3 (Glut-3). A cysteine has been added at the NH2 terminus for coupling to KLH.
