84-1044-03　［取扱停止］GLUT 3 (Glucose Transporter) 100ul　G3900-24A

特徴

• Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
• Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD).
• Individual members of this family have identical predicted secondary structures with 12 transmembrane domains.
• Both N- and C-termini are predicted to be cytoplasmic.
• Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
• GLUT isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
• GLUT 1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
• GLUT 2 provides glucose to the liver and pancreatic cells.
• GLUT 3 is the main transporter in neurons, whereas GLUT 4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL); Detects a major band at ~45kD
• ELISA: 1:10,000-50,000; Coat plates at 1ug/ml with Control Peptide
• Optimal dilutions to be determined by the researcher.
• Control Peptide:G3900-24D: GLUT 3, Human (Glucose Transporter)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.