Glutamate is the main excitatory neurotransmitter in the brain.
To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5.
These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells.
Most recently, vesicular type transporters for glutamate, termed VGLUTs (VGLUT1/BNPI, VGLUT2/DNPI, and EAT-4), have been cloned and characterized that are related to phosphate transporters.
Although neurons exhibit Na-dependent Pi transport, the biological role of Pi uptake is not clear.
Proximal tubules in the kidney reabsorb Pi in the glomeruli by the action of a group of phosphate transporters (Type 1-NaPi related, type 2-NaPi-2 related, and type 3-viral receptor-related.
These receptors show weak (~20% identity) between various subtypes.
A distinct type of brain specific Na+-dependent phosphate (Pi) transporter (BNPI), originally characterized as a plasma membrane transporter has been localized in a subset of glutamatergic neurons (amygdla,cereberal cortex, and hippocmaplus) and identified as VGLUT1 (rat and human 560 aa; ~60kD, ~30% homology with type-1 Pi-transporters).
A novel transporter VGLUT2/DNPI (Differentiation-assocaited Na-Pi cotransporter, ~75% homology with VGLUT1; human/rat/mouse VGLUT2 582 aa) has also been implicated in vesicular glutamate transport.
It is expressed in only a subset of neurons.
It is also localized to synaptic vesicles, at synapses exhibiting classical excitatory features.
VGLUT2 mRNA is found in brain regions that lack VGLUT1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml; Control peptide (Cat #G4005-62D) can be used to coat ELISA plates at 1ug/ml
Western Blot: 1-10ug/ml (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human VGLUT2 at ~65kD.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 18aa within the N-terminus of human VGLUT2 conjugated to KLH. Species Sequence Homolog (95%): mouse, rat
