Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
A band of 95-110kD has been detected in brush border membrane (2)
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The human peptide is 95% conserved in mouse and rat VGLUT2. No significant sequence homology is observed with VGLUT1, EAT-4, or other NaPi-related (Type-1 to Type-III) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：An 18 AA synthetic peptide witin the N-terminus of human VGLUT2 was synthesized, conjugated to KLH
