Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 13, Mol.
Wt.
40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD).
Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT 2 provides glucose to the liver and pancreatic cells.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:10,000.
G3900-15L Control peptide can be used to coat ELISA plates at 1ug/ml
Western Blot: 1-10ug/ml using ECL.
This antibody identified a major band at about 53-61kD in various tissues (1)
Immunohistochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:G3900-15L: GLUT 2, Human Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human GLUT 2.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：A 14aa synthetic peptide sequence near the C-terminal extracellular domain. (KLH). Species Sequence Homology: rat - 64%, mouse - 57%.
