Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, 40-80kD) and sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and C-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Western Blot: 1-10ug/ml using Chemiluminescence technique.
Immunohistochemistry: 2-10ug/mlOptimal dilutions to be determined by the researcher.
Recommended Control Peptide:G3900-15F: GLUT 2, Rat (Glucose Transporter, Insulin Regulatable)
Recommended Tissues:Liver, intestine, kidney, and beta-pancreatic islet cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes rat Glut-2.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Rt
Immunogen：A synthetic peptide corresponding to 16aa near the C-terminal, cytoplasmic domain of rat Glut-2 conjugated with KLH. Subcellular Location: multi-pass membrane protein. Species Sequence Homology: mouse and human-93%, ovine-84%.
